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    Hepatitis-C Investigations

    The clinical symptoms alone cannot determine the diagnosis of Hepatitis C. The diagnostic test for Hepatitis C involves a study of certain Liver enzymes and Hepatitis C specific antibodies in the blood. Anti Hepatitis C (Anti HCV) virus antibodies can be detected in the blood after 3 to 6 weeks. It may be noted that Anti HCV may not be detected adequately during the acute phase of Hepatitis C. ELISA test for HCV antibody is an important diagnostic test to detect the HCV antibodies, which indicates exposure to the infection.

    A. Antibody to Hepatitis C virus:

    1. Enzyme immunoassay test (EIA): This test has to be carried out in the initial stage.

    2. Recombinant Immunoblot Assay (RIBA): This an additional test to support and confirm the diagnosis if the EIA is positive.


    Above tests will give a clue if one has an exposure to the infection. However, they cannot indicate the quantum or extent of infection. There are some quantitative tests to determine the amount of virus titer, that is HCV RNA.

    B. The Quantitative test:
      The quantitative tests to detect amount (titer) of virus (HCV RNA) are:
      Amplicor HCV Monitor™
      Quantiplex HCV RNA (bDNA)
      It is important to note that the laboratory test as above may be false positive or false negative.
    Liver biopsy is not done commonly as a diagnostic test. However, liver biopsy gives an idea about the extent and severity of fibrosis and cirrhosis.
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